尹苗,扶星星,陈希文,王聪,谢作杰,董鹏宇,邓永涛

• 1:四川百诺吉科技有限公司
• 2:四川生猪重大疫病监测与防控工程研究中心

摘要(Abstract)：

猪细小病毒2型（porcine parvovirus type 2,PPV2）是2001年首次在缅甸发现的新型病毒。为深入了解四川省绵阳市PPV2感染及遗传变异情况,使用普通PCR对采自4个地区10个规模化猪场的296份临床病猪血清样本进行了核酸检测,并对其中1份阳性样品进行了全基因组测序和遗传变异分析。结果显示:PPV2检出率为1.69%（5/296）;阳性样品（PPV2-11-MY）全基因组长度为5 506 bp,与12个PPV2参考毒株的核苷酸同源性为93.96%～99.30%,氨基酸同源性为52.50%～73.80%;存在3处氨基酸高变区域,分别位于91～218 aa、513～626 aa和776～1 065 aa;该毒株同源性最接近我国江苏省毒株MW853949.1。本研究不仅丰富了PPV2流行病学数据,为后续疫苗研究提供了理论依据,同时也为进一步研究PPV2致病机理及毒力奠定了基础。

关键词(KeyWords)： 猪细小病毒2型（PPV2）;流行;全基因组;遗传变异分析

Abstract:

Keywords:

基金项目(Foundation): 四川省农业农村厅农业重大技术协同推广计划项目（SCNYTG20-184）;; 四川省科技厅重大科技专项（2023YFN0045）

作者(Author): 尹苗,扶星星,陈希文,王聪,谢作杰,董鹏宇,邓永涛

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