蔡振鸿,陈琼,赵冉,孔繁德,程小文

• 1:厦门市思明区市政管理中心
• 2:厦门市动物疫病预防控制中心
• 3:厦门出入境检验检疫局
• 4:厦门市动物卫生监督所

摘要(Abstract)：

为了解厦门市H9N2亚型禽流感病毒的分子生物学特性,从而为科学防控禽流感提供依据,对2015年的2株H9N2亚型禽流感病毒厦门分离株(A/chicken/Xiamen/09/2015分离自活禽交易市场、A/chicken/Xiamen/10/2015分离自某养殖场)进行全基因序列扩增、测定及分子生物学分析。结果显示:2株分离株的8个基因片段来自3个不同亚群;M、NP基因与安徽、江西、江苏(泰州)H7N9亚型禽流感分离株高度同源;6个基因出现毒力增强,以及与耐药性、哺乳动物嗜性相关的突变。研究结果提示,H9亚型与H7亚型流感病毒间存在基因重组风险,H9N2亚型禽流感病毒的流行会对公共卫生产生潜在安全隐患。

关键词(KeyWords)： 禽流感病毒;H9N2亚型;全基因组测定;序列分析

Abstract:

Keywords:

基金项目(Foundation): 厦门市科技计划项目(3502Z20174050)

作者(Author): 蔡振鸿,陈琼,赵冉,孔繁德,程小文

参考文献(References)：

• [1]GUAN Y,SHORTRIDGE K F,KRAUSS S,et al.Molecularcharacterizationof H9N2 influenza viruses:were they the donors of the“inter-nal”genes of H5N1viruses in Hong Kong?[J].Proceedings of the national academy of sciences of the USA,1999,96(16):9363-9367.
• [2]KAGEYAMA T,FUJISAKI S.Genetic analysis of novel avian A(H7N9)influenza viruses isolated from patients in China,February to April 2013[J].Euro surveill,2013,18(15):20453.
• [3]QI W B.Genesis of the novel human-infecting influenza A(H10N8)virus and potential genetic diversity of the virus inpoultry,China[J].Euro surveillence,2014,19(25):1-13.
• [4]GABRIEL G,DAUBER B,WOLFF T,et al.The viral ploymerase mediates adaptation of an avian influenza virus to a mammalian host[J].Proceedings of the national academy of sciences of the USA,2005,102:18590-18595.
• [5]KATZ J M,LU X H,TUMPEY T M,et al.Molecular correlates of influenza AH5N1 virus pathogenesis in mice[J].Journal of virology,2000,74(22):10807-10810.
• [6]JAKHESARA S J,BHATT V D,PATEL N V,et al.Isolation and characterization of H9N2 influenza virus isolates from poultry respiratory disease outbreak[J].Springer plus,2014,3(1):1-8.
• [7]FAN S,G DENG,J SONG,et al.Two amino acid residues in the matrix protein M1 contrubute to the virulence difference of H5N1 avian influenza a viruses in mice[J].Virology,2009,384:28-32.
• [8]LI Z,JIANGY,JIAO P,et al.The NS 1 gene contributes to the virulence of H5N1 avian influenza viruses[J].Journal of virology,2006,80:11115-11123.
• [9]龙进学,薛峰,彭宜,等.H5N1亚型禽流感病毒NS第263-277位核苷酸缺失提高病毒对鸡的致病力[J].微生物学报,2006,46(2):301-305.
• [10]LONG J X,PENG D X,LIU Y L,et al.Virulence of H5N1 avian influenza virus enhanced by a 15-nucleotide deletion in the viral nonstructural gene[J].Virus genes,2008,36(3):471-478.
• [11]WANG S,SHI W M,MWEENE A,et al.Genetic analysis of the nonstructural(NS)genes of H9N2chicken influenza viruses isolated in china during 1998—2002[J].Virus genes,2005,31:329-335.
• [12]HAYDEN F G.Amantadine and rimantadine-clinical aspects[M].New York:John Wiley&Sons Ltd,1996:59-77.
• [13]YAVARIAN J,AZAD T M,ZHENG X,et al.Amantadine resistance in relation to the evolution of influenza A(H3N2)viruses in Iran[J].Antiviral research,2010,88(2):193-196.