零售鸡肉中沙门氏菌生长预测模型的建立Establishment of Growth Prediction Model for Salmonella in Retail Chicken
孙婷婷,赵格,宋雪,赵建梅,刘娜,王娟,李月华,黄秀梅,王君玮,邹明
摘要(Abstract):
为了建立零售鸡肉中沙门氏菌的生长预测模型,以在超市购买的新鲜鸡肉和肠炎沙门氏菌作为研究对象,选择4、10、16、25、30、37℃条件,对肠炎沙门氏菌在零售鸡肉中的生长情况进行研究,绘制生长曲线。对一级模型,用Curve Expert软件中的Gompertz、Richards、Logistic 3种模型和DMFit软件中的Baranyi模型进行拟合,以确定最适用模型。4种模型拟合后的Baranyi模型相关系数都在0.98以上。数据表明该模型拟合程度最好,最适合预测沙门氏菌在鸡肉中的生长动态。二级模型是将一级模型拟合的数据带入Ratkowsky方程。此方程描述的是温度对最大生长速率的影响。通过准确因子、偏差因子以及均方根误差,对模型的准确性进行检验。结果显示模型的准确因子为1.129 698,偏差因子为0.984 85,均方根误差为0.091 5,决定系数R2为0.982 5,说明建立的模型可靠性高。
关键词(KeyWords): 沙门氏菌;零售鸡肉;预测微生物学;生长模型
基金项目(Foundation): 国家农产品质量安全风险评估项目计划(GJFP201600703、GJFP201700703);; 科技部科技基础性工作专项(2012FY111000)
作者(Author): 孙婷婷,赵格,宋雪,赵建梅,刘娜,王娟,李月华,黄秀梅,王君玮,邹明
参考文献(References):
- [1]GOULD L H,WALSH K A,VIEIRA A R,et al.Surveillance for foodborne diseaks:United States,1998-2008[J].Morbidity and mortality weekly report,2013,62(2):1-34.
- [2]孙长颢.营养与食品卫生学[M].北京:人民卫生出版社,2012:447-448.
- [3]YANG B,XI M,WANG X,et al.Prevalence of Salmonella on raw poultry at retail markets in China[J].Journal of food protection,2011,74(10):1724-1728.
- [4]赵瑞兰.沙门氏菌预测模型的建立及出口分割鸡肉中沙门氏菌的风险分析[D].泰安:山东农业大学,2005.
- [5]王军,郑增忍.动物源性食品中沙门氏菌的风险评估[J].中国动物检疫,2007,24(4):23-25.
- [6]DING T,SHIM Y H,CHOI N J,et a1.Mathematical modeling on the growth of Staphy lococcus aureus in sandwich[J].Food science biotechnology,2010,19(3):763-768.
- [7]SWINNEN I A M,BERNAERTS K,DENS E J J,et al.Predictive modelling of the microbial lag phase:a review[J].International journal of food microbiology,2004,94(2):137-159.
- [8]FERRER J,PRATS C,LOPEZ D,et a1.Mathematical modelling methodologies in predictive food microbiology:a SWOT analysis[J].International journal of food microbiology,2009,134(1/2):2-8.
- [9]郭剑飞,李柏林,欧杰.预测微生物学数学建模的方法构建[J].食品科学,2004,25(11):53-57.
- [10]RATKOWSKY D A,OLLEY J,MCMEEKIN T A,et al.Relationship between temperature and growth rate of bacterial culture[J].Bacterial,1982,149(1):1-5.
- [11]吴云凤,袁宝君.南京市零售生鲜鸡类食品中沙门氏菌的污染状况调查[J].南京医科大学学报,2015,35(2):284-287.
- [12]吴云凤,袁宝君.零售鸡肉中沙门氏菌的半定量风险评估研究[J].食品安全质量检测学报,2014,12(5):4157-4162.
- [13]张玉.山东省肉鸡中沙门氏菌的定量风险评估[D].济南:山东大学,2015.
- [14]MANN J E,SMITH L,BRASHEARS M M.Validation of time and temperature values as critical limits for Slamonella and background flora growth during the production of fresh gound and boneless pork products[J].Journal of food protection,2004,67(7):1389-1393.
- [15]JUNEJA V K,MELENDRES M V,HUANG L,et al.Modeling the effect of temperature on growth of Salmonella in chicken[J].Food microbiology,2007,24(4):328-335.
- [16]SABIKE I,FUJIKAWA H,EDRIS1 A M,et al.Evaluation of predicting performances of extended logistic model and baranyi model forthe growth of salmonella enteritidis in minced beef[J].Benha veterinary medical journal,2014,27(2):91-103.